The deciding clash of the Virgin Australia International Rules Series between Australia and Ireland on November 18 will be the farewell match at Domain Stadium.
The AFL has confirmed that the second Test will be played at the soon-to-be-defunct Perth venue on a Saturday evening.
Bouncedown will be at 4.45pm local time, or 7.45pm AEDT.
Adelaide Oval will host the first IRS clash at 3.30pm local time (4pm AEDT) on Sunday, November 12.
It will be the first time since 2013 that the series will feature two Tests.
Ireland holds the Cormac McAnallen Trophy after defeating Australia in the one-off Test at Croke Park in 2015.
Geelong coach Chris Scott and Fremantle mentor Ross Lyon were assistants to Hawthorn mastermind Alastair Clarkson for that series.
This year, Scott will step up as senior coach, with support from his brother Brad and Lyon.
The last time Perth hosted a Test was 2014, with Australia winning 56-46.
"Another Test in Perth, which is fantastic, a rich history here, a number of victories, close games and sell-outs," Lyon said.
"My experience here was a sell-out and previous to that every other game has been a sell-out, which is fantastic, and it really displays the passion of Perth football people.
"Added to that is the sentiment of the last AFL game at Domain Stadium after a long period of time, so there's a lot to like."
To be available for selection, AFL players must have been named All Australian at some point in their careers.
Fremantle skipper Nat Fyfe is keen to finally represent his country after injuring his shoulder during a practice game before the 2014 series.
"I was extremely disappointed to miss out," he said. "That's really what invigorated me to play again when the opportunity came up this year.
"For me leadership-wise, watching the best in the business how they mingle with our players, on-field leadership, how they engage and interact, so eyes were wide-open then and they will be this time around as well."
